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008			141201s2015 xxu| s |||| 0|eng d
020			_a9781493922055
024	7		_a10.1007/978-1-4939-2205-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aInsoluble Proteins _bMethods and Protocols _cedited by Elena García-Fruitós.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XVI, 425 páginas) _b72 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1258
505	0		_aGeneral Introduction: Recombinant Protein Production and Purification of Insoluble Proteins -- Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production -- Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility -- Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification -- Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them -- Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates -- Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems -- Functional Expression of Plant Membrane Proteins in Lactococcus lactis -- High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of "Difficult-to-Express" Secretory Recombinant Proteins -- Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins -- Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins -- Recombinant Glycoprotein Production in Human Cell Lines -- Soluble Recombinant Protein Production in Pseudoaltermonas haloplanktis TAC125 -- A Screening Methodology for Purifying Proteins with Aggregation Problems -- Solubilization and Refolding of Inclusion Body Proteins -- Bacterial Inclusion Body Purification -- Characterization of Intracellular Aggresomes by Fluorescent Microscopy -- Dialysis: A Characterization Method of Aggregation Tendency -- Applications of Mass Spectrometry to the Study of Protein Aggregation -- Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy -- Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR) -- Methods for Characterization of Protein Aggregates -- Predicting the Solubility of Recombinant Proteins in Escherichia coli -- Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.
520			_aWith insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.
700	1		_aGarcía-Fruitós, Elena _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493922048
776	0	8	_iPrinted edition: _z9781493922062
776	0	8	_iPrinted edition: _z9781493946754
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2205-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234278 _d234278