000			03426nam a22003615i 4500
001			234280
003			ES-VaUE
005			20221220020553.0
007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592597499
024	7		_a10.1385/1592597491 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNitric Oxide Protocols _cedited by M. A Titheradge.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XI, 324 páginas) _b40 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v100
505	0		_aEnzymology of Nitric Oxide Synthases -- Purification of the Inducible Nitric Oxide Synthase -- Purification of the Constitutive Nitric Oxide Synthase -- Cloning and Expression of Human Inducible Nitric Oxide Synthase -- Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System -- Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO -- Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation -- Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation -- The Enzymatic Measurement of Nitrate and Nitrite -- Determination of NOS Activity Using Cyclic-GMP Formation -- Determination of NO with a Clark-Type Electrode -- The Measurement of NO in Biological Systems Using Chemiluminescence -- Measurement of NO Using Electron Paramagnetic Resonance -- Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction -- Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay -- Detection of NOS Isoforms by Western-Blot Analysis -- Immunohistochemical Localization of NOS Isoforms -- A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization -- Use of NO Donors in Biological Systems -- Making and Working with Peroxynitrite -- The Use of NO Gas in Biological Systems -- A Microtiter-Plate Assay of Human NOS Isoforms -- Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings -- Measurement of Biopterin and the Use of Inhibitors of Biopterin Biosynthesis -- A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I -- Simultaneous Measurement of Mitochondrial Function and NO -- Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen -- The Determination of Nitrotyrosine Residues in Proteins -- Measurement of DNA Damage Using the Comet Assay -- Methods for the Study of NO-Induced Apoptosis in Cultured Cells.
700	1		_aTitheradge, M. A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942968
776	0	8	_iPrinted edition: _z9781489942951
776	0	8	_iPrinted edition: _z9780896034709
776	0	8	_iPrinted edition: _z9780896035379
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597491 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c234280 _d234280