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020 _a9781592594993
024 7 _a10.1385/0896032299
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
100 1 _aBurmeister, Margit.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aPulsed-Field Gel Electrophoresis
_bProtocols, Methods, and Theories
_cby Margit Burmeister, Levy Ulanovsky.
250 _a1st edition 1992
264 1 _aTotowa, NJ
_bHumana Press
_c1992
300 _a1 recurso en línea (XIII, 481 páginas)
_b99 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v12
505 0 _aElectrophoretic Techniques -- Field-Inversion Gel Electrophoresis -- Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF) -- Separation and Size Determination of DNA over a 10-,200 kbp Range -- PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE) -- Transverse Alternating-Field Electrophoresis -- Rotating Field Gel Electrophoresis (ROFE) -- Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE) -- Auxiliary Methods -- Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis -- Encapsulation of Cells in Agarose Beads -- Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE) -- Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis -- Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases -- Desktop Digital Imaging -- Biological Applications -- Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE) -- Protozoan Genomes -- Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis -- Analysis of Yeast Artificial Chromosome Clones -- Strategies for Mapping Large Regions of Mammalian Genomes -- Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA -- Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly Repeated DNA -- Construction of Lambda Libraries from Large PFGE Fragments -- In-Gel Detection of DNA -- Theories and Observations -- Theories of Pulsed-Field Gel Electrophoresis -- The Dynamics of DNA Gel Electrophoresis -- Orientation of the Agarose Matrix by Pulsed Electric Fields -- Reptation Theory of Pulsed Electrophoresis and Trapping Electrophoresis -- Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).
700 1 _aUlanovsky, Levy.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
776 0 8 _iPrinted edition:
_z9781489943910
776 0 8 _iPrinted edition:
_z9781489943903
776 0 8 _iPrinted edition:
_z9780896032293
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032299
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1992
999 _c234286
_d234286