000			03639nam a22003615i 4500
001			234290
003			ES-VaUE
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007			cr nn 008mamaa
008			111011s2012 xxu| s |||| 0|eng d
020			_a9781617793523
024	7		_a10.1007/978-1-61779-352-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Expression in Mammalian Cells _bMethods and Protocols _cedited by James L Hartley.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 276 páginas) _b48 ilustraciones, 9 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v801
505	0		_aWhy Proteins in Mammalian Cells? -- Large Scale Transfection of Mammalian Cells -- Selection of High Expressing Mammalian Cells by Surface Display of Reporters -- Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles -- Transfection of Difficult-to-Transfect Primary Mammalian Cells -- Stable Protein Expression in Mammalian Cells Using Baculoviruses -- Using Matrix Attachment Regions to Improve Recombinant Protein Production -- Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells -- Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells -- Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells -- Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker -- Tools for Co-Producing Multiple Proteins in Mammalian Cells -- Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases -- Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA -- Methods for Constructing Clones for Protein Expression in Mammalian Cells -- Optimizing Transient Recombinant Protein Expression in Mammalian Cells.
520			_aThrough all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease.  In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.   Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
700	1		_aHartley, James L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617793516
776	0	8	_iPrinted edition: _z9781617793530
776	0	8	_iPrinted edition: _z9781493961603
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-352-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c234290 _d234290