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008			150304s2015 xxu| s |||| 0|eng d
020			_a9781493924950
024	7		_a10.1007/978-1-4939-2495-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSalamanders in Regeneration Research _bMethods and Protocols _cedited by Anoop Kumar, András Simon.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 357 páginas) _b89 ilustraciones, 81 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1290
505	0		_aVariation in Salamanders: An Essay on Genomes, Development, and Evolution -- Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research -- Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl -- Husbandry of Spanish Ribbed Newts (Pleurodeles waltl -- Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies -- Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH -- Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation -- The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration -- High-efficiency Electroporation of the Spinal Cord in Larval Axolotl -- Pseudotyped Retroviruses for Infecting Axolotl -- Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum) -- Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos -- In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration -- Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas -- Culture and Transfection of Axolotl Cells -- Isolation and Culture of Neurospheres from the Adult Newt Brain -- Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb -- Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture --  Long Term Organ Cultures of Newt Hearts -- In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration -- Transgenesis in Axolotl (Ambystoma mexicanum) -- Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA‐guided Nuclease -- Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl -- Transcriptomics Using Axolotls -- Sal-Site: Research Resources for The Mexican Axolotl -- Data Mining in Newt-omics, the Repository for Omics Data from the Newt.
520			_aThis detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.
700	1		_aKumar, Anoop _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSimon, András _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493924943
776	0	8	_iPrinted edition: _z9781493924967
776	0	8	_iPrinted edition: _z9781493949472
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2495-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234292 _d234292