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020 _a9781597453356
024 7 _a10.1007/978-1-59745-335-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCancer Genomics and Proteomics
_bMethods and Protocols
_cedited by Paul B. Fisher.
250 _a1st edition 2007
264 1 _aTotowa, NJ
_bHumana Press
_c2007
300 _a1 recurso en línea (XVI, 358 páginas)
_b60 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v383
505 0 _aReciprocal Subtraction Differential RNA Display (RSDD) -- Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH) -- The Application of Differential Display as a Gene Profiling Tool -- Serial Analysis of Gene Expression (SAGE) -- Gene Expression Profile Analysis of Tumors -- cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-?B Related Genes in Squamous Cell Carcinoma -- Gene Profiling Uncovers Retinoid Target Genes -- Complete Open Reading Frame (C-ORF) Technique -- Chromatin Immunoprecipitation Assays -- Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection -- Molecular Cytogenetic Applications in Analysis of the Cancer Genome -- Monitoring Methylation and Gene Expression in Cancer -- The Use of Phage Display Peptide Libraries for Basic and Translational Research -- Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions -- Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated With Tumor Growth Arrest -- Surface-Epitope Masking (SEM) -- Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells -- PKR in Innate Immunity, Cancer, and Viral Oncolysis -- Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells -- Analysis of Growth Properties and Cell Cycle Regulation Using Mouse Embryonic Fibroblast Cells -- Reverse Phase Protein Microarrays for Monitoring Biological Responses -- Protein Crystallization.
520 _aCancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects. Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization. Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.
700 1 _aFisher, Paul B
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617376054
776 0 8 _iPrinted edition:
_z9781588295040
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-335-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2007
999 _c234295
_d234295