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020 _a9781617792892
024 7 _a10.1007/978-1-61779-289-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGene Expression Profiling
_bMethods and Protocols
_cedited by Lorraine O'Driscoll.
250 _a2nd edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (X, 200 páginas)
_b37 ilustraciones, 15 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v784
505 0 _aReal-Time PCR and Multiplex Approaches -- Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs -- Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals -- Software and Tools for Microarray Data Analysis -- Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells -- Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay -- MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs -- Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases -- 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins -- Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis -- Immunohistochemical and Immunofluorescence Procedures for Protein Analysis -- Advanced Microscopy: Laser Scanning Confocal Microscopy -- Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling -- Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties.
520 _aUnderstanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.  In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.
700 1 _aO'Driscoll, Lorraine
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617792885
776 0 8 _iPrinted edition:
_z9781617792908
776 0 8 _iPrinted edition:
_z9781493961733
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-289-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c234317
_d234317