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001			234318
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008			131016s2014 xxu| s |||| 0|eng d
020			_a9781627036795
024	7		_a10.1007/978-1-62703-679-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAdenovirus _bMethods and Protocols _cedited by Miguel Chillón, Assumpció Bosch.
250			_a3rd edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 247 páginas) _b37 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1089
505	0		_aBiophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle -- Proteome Analysis of Adenovirus Using Mass Spectrometry -- Capsid Modification Strategies for Detargeting Adenoviral Vectors -- Use of Dodecahedron "VLPs" as an Alternative to the Whole Adenovirus -- Study of Adenovirus and CAR Axonal Transport in Primary Neurons -- The Use of Chromatin Immunoprecipitation (ChIP) to Study the Binding of Viral Proteins to the Adenovirus Genome In Vivo -- DNA Microarray to Analyze Adenovirus-Host Interactions -- Determination of the Transforming Activities of Adenovirus Oncogenes -- Oncolytic Adenovirus Characterization: Activity and Immune Responses -- The Analysis of Innate Immune Response to Adenovirus Using Antibody Arrays -- Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology -- Construction, Production, and Purification of Recombinant Adenovirus Vectors -- Scalable Production of Adenovirus Vectors -- Canine Adenovirus Downstream Processing Protocol -- Production of High-Capacity Adenovirus Vectors -- Production of Chimeric Adenovirus.
520			_aReflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics.  Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.
700	1		_aChillón, Miguel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBosch, Assumpció _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627036801
776	0	8	_iPrinted edition: _z9781627036788
776	0	8	_iPrinted edition: _z9781493963256
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-679-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234318 _d234318