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008			160727s2016 xxu| s |||| 0|eng d
020			_a9781493936878
024	7		_a10.1007/978-1-4939-3687-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHuman Respiratory Syncytial Virus _bMethods and Protocols _cedited by Ralph A. Tripp, Patricia A. Jorquera.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 247 páginas) _b39 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1442
505	0		_aHuman Respiratory Syncytial Virus: An Introduction -- RSV Growth and Quantification by Microtitration and qRT-PCR Assays -- Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay -- Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays -- Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells -- Development of Human Monoclonal Antibodies against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique -- Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection -- Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to Probe Functional Importance -- In Vitro Modelling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs) -- Reverse Genetics of Respiratory Syncytial Virus -- Use of Minigenome Systems to Study RSV Transcription -- Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics -- A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets -- MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples -- Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus -- In Vivo Assessment of Airway Function in the Mouse Model -- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus. .
520			_aThis volume summarizes current techniques; ranging from protocols for virus growth, isolation, quantification and generation of recombinant RSV virus to procedures for the efficient characterization of the host immune response to RSV infection. These techniques are used in numerous laboratories around the world and are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Humana Respiratory Syncytial Virus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aTripp, Ralph A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aJorquera, Patricia A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936854
776	0	8	_iPrinted edition: _z9781493936861
776	0	8	_iPrinted edition: _z9781493981175
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3687-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234320 _d234320