000			04385nam a22003615i 4500
001			234321
003			ES-VaUE
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007			cr nn 008mamaa
008			160811s2016 xxu| s |||| 0|eng d
020			_a9781493937462
024	7		_a10.1007/978-1-4939-3746-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Tyrosine Phosphatases _bMethods and Protocols _cedited by Rafael Pulido.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 402 páginas) _b79 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1447
505	0		_aThe Extended Family of Protein Tyrosine Phosphatases -- Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome -- Expression, Purification, and Kinetic Analysis of PTP Domains -- Peptide Microarrays for Real Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples -- Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B -- Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates -- Assessing the Biological Activity of the Glucan Phosphatase Laforin -- Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents -- Analyzing Pseudophosphatase Function -- Crystallization of PTP Domains -- NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases -- Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs) -- In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions -- Use of Dominant Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates -- Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains -- Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling -- Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Haemostasis -- Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis -- Studying Protein-Tyrosine Phosphatases in Zebrafish -- Live staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands -- Methods to Study Protein Tyrosine Phosphatases Acting on Yeast MAPKs.
520			_aThis book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. < Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
700	1		_aPulido, Rafael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493937448
776	0	8	_iPrinted edition: _z9781493937455
776	0	8	_iPrinted edition: _z9781493981274
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3746-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234321 _d234321