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001			234322
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007			cr nn 008mamaa
008			170505s2017 xxu| s |||| 0|eng d
020			_a9781493968770
024	7		_a10.1007/978-1-4939-6877-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTh9 Cells _bMethods and Protocols _cedited by Ritobrata Goswami.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 259 páginas) _b29 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1585
505	0		_aTh9 Cells: New Member of T Helper Cell Family -- IL-9: Function, Sources, and Detection -- IL-9 Signaling Pathway: An Update -- A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells -- T Cell Receptor and Co-Stimulatory Signals for Th9 Generation -- Polarizing Cytokines for Human Th9 Cell Differentiation -- Determining the Frequencies of Th9 Cells from Whole Blood -- IL-9 Production by Nonconventional T Helper Cells -- Prediction and Validation of Transcription Factors Binding Sites in the IL-9 Locus -- Flow Cytometric Assessment of STAT Molecules in Th9 Cells -- Transcription Factors Downstream of IL-4 and TGF- β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry -- Retroviral Transduction and Reporter Assay: Transcription Factor Co-Operation in Th9 Cell Development -- Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid-Luminescent DNA-Precipitation Assay -- Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation -- Allergic Inflammation and Atopic Disease: Role of Th9 Cells -- Characterization of T Helper 9 (Th9) Cells in the Development of EAE and IBD -- B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer -- Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models -- Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings.
520			_aThis volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Th9 Cells: Methods and Protocols is a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events.
700	1		_aGoswami, Ritobrata _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968763
776	0	8	_iPrinted edition: _z9781493968787
776	0	8	_iPrinted edition: _z9781493983261
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6877-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234322 _d234322