000			04428nam a22003735i 4500
001			234327
003			ES-VaUE
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007			cr nn 008mamaa
008			141006s2015 xxu| s |||| 0|eng d
020			_a9781493917433
024	7		_a10.1007/978-1-4939-1743-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Virology Protocols _bNew Approaches to Detect Viruses and Host Responses _cedited by Ichiro Uyeda, Chikara Masuta.
250			_a3rd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 292 páginas) _b70 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1236
505	0		_aDetection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method -- RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers -- New Method to Isolate Total dsRNA -- Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses -- Detection Methods for Rice Viruses by a Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) -- Real-Time PCR Protocols for the Quantification of the Begomovirus Tomato Yellow Leaf Curl Sardinia Virus in Tomato Plants and in Its Insect Vector -- Detection and Analysis of Non-Retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes -- Detection of Plant Viruses in Natural Environments by Using RNA-Seq -- Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA -- Drawing siRNAs of Viral Origin out from Plant siRNAs Libraries -- Viral Detection by High-Throughput Sequencing -- Analysis and Application of Viroid-Specific Small RNAs Generated by Viroid-Inducing RNA Silencing -- Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing -- SuperSAGE as an Analytical Tool for Host and Viral Gene Expression -- Microarray Analysis of R-Gene-Mediated Resistance to Viruses -- Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus -- Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors -- Simplified Methods for the Construction of RNA and DNA Virus Infectious Clones -- Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control -- Detection of Plant Virus in Meristem by Immunohistochemistry and In Situ Hybridization.
520			_aPlant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.
700	1		_aUyeda, Ichiro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMasuta, Chikara _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493917440
776	0	8	_iPrinted edition: _z9781493917426
776	0	8	_iPrinted edition: _z9781493955404
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1743-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234327 _d234327