000			03509nam a22003615i 4500
001			234336
003			ES-VaUE
005			20221220020556.0
007			cr nn 008mamaa
008			180208s2018 xxu| s |||| 0|eng d
020			_a9781493975464
024	7		_a10.1007/978-1-4939-7546-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSchizosaccharomyces pombe _bMethods and Protocols _cedited by Teresa L. Singleton.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 244 páginas) _b48 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1721
505	0		_aPreparation of Solutions and Reagents -- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing -- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe -- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs -- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast -- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast -- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis -- The No-nonsens SDS-PAGE -- Crystallization of Recombinant α-actinin and Related Proteins -- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy -- Antibody Pull-down Experiments in Fission Yeast -- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation -- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo -- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe -- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast -- Random Spore Analysis in Fission Yeast -- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains -- Schizosaccharomyces pombe Biotechnological Applications in Winemaking -- Schizosaccharomyces pombe Isolation Protocol. .
520			_aThis volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.
700	1		_aSingleton, Teresa L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493975457
776	0	8	_iPrinted edition: _z9781493975471
776	0	8	_iPrinted edition: _z9781493985166
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7546-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234336 _d234336