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008			180713s2018 xxu| s |||| 0|eng d
020			_a9781493978625
024	7		_a10.1007/978-1-4939-7862-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMyelin _bMethods and Protocols _cedited by Ashwin Woodhoo.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 294 páginas) _b70 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1791
505	0		_aElectron Microscopy for the Analysis of Peripheral Nerve Myelin -- Immunohistochemical Analysis of Myelin Structures -- Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish -- Lipidomics Profiling of Myelin -- Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry -- Proteomic Analysis of Myelin Composition -- Isolation and Purification of Primary Rodent Schwann Cells -- Isolation, Expansion, and Maturation of Oligodendrocyte Lineage Cells Obtained from Rat Neonatal Brain and Optic Nerve -- DRG Neuron/Schwann Cells Myelinating Co-Cultures -- Neuron/Oligodendrocyte Myelination Co-Culture -- Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination -- Modeling PNS and CNS Myelination Using Microfluidic Chambers -- Modeling CNS Myelination Using Micropillar Arrays -- A Central Nervous System Axonal Myelination Assay for High-Throughput Screening -- Analyzing Autophagic Flux in Nerve Cultures -- Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro -- Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOD35-55 in the Mouse -- Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin -- Myelin Protein Zero180-199 Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice -- Transection and Crush Models of Nerve Injury to Measure Repair and Remyelination in Peripheral Nerve -- In Vivo Introduction of Transgenes into Mouse Sciatic Nerve Cells Using Viral Vectors -- Intrathecal Delivery of Viral Vectors for Gene Therapy -- Efficient Gene Transfer to Myelinating Schwann Cells in the Rodent Sciatic Nerve Using In Vivo Electroporation.
520			_aThis volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field. Chapters in this book are divided into three parts: Part One focuses on visualizing myelin and studying it on a biochemical level; Part Two looks at cell culture systems used to uncover key mechanisms that regulate formation and maintenance of myelin; and Part Three discusses modeling disorders of myelin in both CNS and PNS, and therapeutic strategies for targeting these disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Myelin: Methods and Protocols is a valuable resource for physiologists, molecular biologists, anatomists, cell and developmental biologists, and physician-scientists working in the field of myelin biology and the disorders associated with it.
700	1		_aWoodhoo, Ashwin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978618
776	0	8	_iPrinted edition: _z9781493978632
776	0	8	_iPrinted edition: _z9781493993079
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7862-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234345 _d234345