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008			150730s2016 xxu| s |||| 0|eng d
020			_a9781493927630
024	7		_a10.1007/978-1-4939-2763-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNucleic Acid Crystallography _bMethods and Protocols _cedited by Eric Ennifar.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 356 páginas) _b116 ilustraciones, 98 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1320
505	0		_aPerspectives and Pitfalls in Nucleic Acids Crystallography -- Preparation of Short 5'-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies -- Preparation and Crystallization of Riboswitches -- In Vitro/In Vivo Production of tRNA for X-Ray Studies -- Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA -- Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs -- Fab Chaperone-Assisted RNA Crystallography (Fab CARC) -- Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes -- Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes -- Crystallographic Data and Model Quality -- Advanced Crystallographic Data Collection Protocols for Experimental Phasing -- Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases -- Practical Radiation Damage Induced Phasing -- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data -- Using Molecular Replacement Phasing to Study the Structure and Function of RNA -- Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution? -- RNA Structure Refinement Using the ERRASER-Phenix Pipeline -- Neutron Nucleic Acid Crystallography -- Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins -- Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers -- Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor -- Anions in Nucleic Acid Crystallography.
520			_aThis volume includes comprehensive and up-to-date coverage of all nucleic acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.  .
700	1		_aEnnifar, Eric _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493927623
776	0	8	_iPrinted edition: _z9781493927647
776	0	8	_iPrinted edition: _z9781493946419
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2763-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234346 _d234346