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020			_a9781592590827
024	7		_a10.1385/1592590829 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCeliac Disease _bMethods and Protocols _cedited by Michael N. Marsh.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XVI, 288 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v41
505	0		_aCeliac Disease -- Genotyping Methodologies -- From Linkage to Genes -- Linkage and the Transmission Disequilibrium Test in Complex Traits -- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats -- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry -- Synthetic Peptide Libraries for T-Cell Epitope Identification -- Characterization of HLA-DQ-Specific Peptide-Binding Motifs -- Studies of Gliadin-Specific T-Cells in Celiac Disease -- Morphometric Analysis of Intestinal Mucosa -- Morphometry of Rectal Mucosa -- Organ Culture of Rectal Mucosa -- In Situ Hybridization -- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease -- Immunohistochemistry in Research and Diagnosis of Celiac Disease -- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease -- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease -- Whole Gut Lavage Fluid Analysis.
520			_aWith the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.
700	1		_aMarsh, Michael N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371158
776	0	8	_iPrinted edition: _z9780896036505
776	0	8	_iPrinted edition: _z9781489941862
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590829 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c234347 _d234347