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001			234352
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007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597455497
024	7		_a10.1007/978-1-59745-549-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aReporter Genes _bA Practical Guide _cedited by Don Anson.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (X, 158 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v411
505	0		_aMethodologies for Staining and Visualisation of ?-Galactosidase in Mouse Embryos and Tissues -- Immunohistochemical Detection of ?-Galactosidase or Green Fluorescent Protein on Tissue Sections -- Detection of Reporter Gene Expression in Murine Airways -- Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques -- Fluorescent Proteins for Cell Biology -- Detection of GFP During Nervous System Development in Drosophila melanogaster -- Autofluorescent Proteins for Flow Cytometry -- Fluorescent Protein Reporter Systems for Single-Cell Measurements -- Subcellular Imaging of Cancer Cells in Live Mice -- Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects -- Green Fluorescent Protein as a Tracer in Chimeric Tissues.
520			_aReporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
700	1		_aAnson, Don _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781588297396
776	0	8	_iPrinted edition: _z9781627038621
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-549-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c234352 _d234352