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001			234353
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007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592592661
024	7		_a10.1385/159259266X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aFlavoprotein Protocols _cedited by Steven K. Chapman, Graeme A. Reid.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XII, 256 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v131
505	0		_aUV-Visible Spectroscopy as a Tool to Study Flavoproteins -- Identifying and Quantitating FAD and FMN in Simple and in Iron-Sulfur-Containing Flavoproteins -- Fluorescence Analysis of Flavoproteins -- Potentiometric Measurement of Oxidation-Reduction Potentials -- Flavoprotein Kinetics -- Application of Freeze Quenching to the Study of Rapid Reactions in Flavoproteins -- Application of Electron Spin Resonance (ESR) for Detection and Characterization of Flavoprotein Semiquinones -- Circular Dichroism Studies of Flavoproteins -- Vibrational Spectroscopy of Flavoproteins -- NMR of Flavoproteins -- Flavoprotein Resolution and Reconstitution -- Flavoenzyme Structure and Function -- Identification of Covalent Flavoproteins and Analysis of the Covalent Link -- Flavin Synthesis and Incorporation into Synthetic Peptides -- Computational Methods in Flavin Research -- Flavins and Flavoenzymes in Diagnosis and Therapy.
520			_aAs a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow-or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
700	1		_aChapman, Steven K _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aReid, Graeme A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371585
776	0	8	_iPrinted edition: _z9781489941756
776	0	8	_iPrinted edition: _z9780896037342
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259266X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c234353 _d234353