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020 _a9781617791086
024 7 _a10.1007/978-1-61779-108-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMammalian Cell Viability
_bMethods and Protocols
_cedited by Martin J. Stoddart.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (XII, 240 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v740
505 0 _aCell Viability Assays: Introduction -- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods -- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay -- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells -- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye -- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability -- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays -- Annexin V / 7-AAD Staining in Keratinocytes -- Measurement of Caspase Activity: From Cell Populations to Individual Cells -- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes -- Multiplexing Cell Viability Assays -- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death -- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs -- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers -- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections -- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay -- Exploiting the Liberation of Zn2+ to Measure Cell Viability -- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs -- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test -- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses -- Digital Image Processing of Live/Dead Staining.
520 _aWhether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.   Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
700 1 _aStoddart, Martin J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617791079
776 0 8 _iPrinted edition:
_z9781617791093
776 0 8 _iPrinted edition:
_z9781493961535
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-108-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c234361
_d234361