000			03020nam a22003495i 4500
001			234364
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1997 xxu| s |||| 0|eng d
020			_a9781592596355
024	7		_a10.1385/0896034151 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRegulatory Protein Modification _bTechniques and Protocols _cedited by Hugh C. Hemmings.
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XIV, 446 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v30
505	0		_aAnalysis of Protein Phosphorylation in Intact Cells and Extracts -- Analysis of Protein Dephosphorylation in Intact Cells and Extracts -- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes) -- Protein Kinase and Phosphatase Inhibitors -- Phosphorylation State-Specific Antibodies -- Protein Tyrosine Phosphorylation -- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis -- Protein methylation in the nervous system -- Long-Chain Fatty Acylation of Proteins -- Protein ADP-Ribosylation -- Glycosylation and Glycosylphosphatidylinositol Membrane Anchors.
520			_aHugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today. Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.
700	1		_aHemmings, Hugh C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617370304
776	0	8	_iPrinted edition: _z9780896034150
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034151 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1997
999			_c234364 _d234364