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020 _a9781493917143
024 7 _a10.1007/978-1-4939-1714-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGlycosaminoglycans
_bChemistry and Biology
_cedited by Kuberan Balagurunathan, Hiroshi Nakato, Umesh R. Desai.
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XVI, 625 páginas)
_b140 ilustraciones, 72 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1229
505 0 _aAutomated Synthesis Of Chondroitin Sulfate Oligosaccharides -- Enzymatic Synthesis Of Heparan Sulfate And Heparin -- Production Of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides By Enzymatic Depolymerization -- Chemical Modification Of Heparin And Heparosan -- Synthesis Of Sulfur Isotope Labeled Sulfate Donor, 3'-Phosphoadenosine-5'-Phosphosulfate, For Studying Glycosaminoglycan Functions -- Preparation Of Isotope Enriched Heparan Sulfate Precursors For Structural Biology Studies -- Synthesis Of Glycosaminoglycan Mimetics Through Sulfation Of Polyphenols -- Synthesis Of Selective Inhibitors Of Heparan Sulfate And Chondroitin Sulfate Proteoglycan Biosynthesis -- Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical and Spectroscopic Analysis -- Human Blood Glycosaminoglycans: Isolation and Analysis -- Chromatographic Molecular Weight Measurements For Heparin, Its Fragments And Fractions, and Other Glycosaminoglycans -- Mass Spectrometric Methods For The Analysis Of Heparin And Heparan Sulfate -- Validated Capillary Electrophoretic Assays For Disaccharide Composition Analysis Of Galactosaminoglycans In Biologic Samples and Drugs/Neutraceuticals -- Fast Screening Of Glycosaminoglycan Disaccharides By Fluorophore Assisted Carbohydrate Electrophoresis (Face): Applications To Biologic Samples And Pharmaceutical Formulations -- Capillary Electrophoretic Analysis Of Isolated Sulfated Polysaccharides To Characterize Pharmaceutical Products -- Methods For Measuring Exchangeable Protons In Glycosaminoglycans -- Heparan Sulfate Structure: Methods To Study N-Sulfation and Ndst Action -- Analysis Of Hyaluronan Synthase Activity -- A Rapid, Non-Radioactive Assay For Measuring Heparan Sulfate C-5 Epimerase Activity Using -- Aggrecan - Approaches To Study Biophysical And Biomechanical Properties -- Use Of Flow Cytometry For Characterisation And Fractionation Of Cell Populations Based On Their Expression Of Heparan Sulfate Epitopes -- A Transgenic Approach To Live Imaging Of Heparan Sulfate Modification Patterns -- Informatics Tools To Advance The Biology Of Glycosaminoglycans and Proteoglycans -- Designing 'High-Affinity, High-Specificity' Glycosaminoglycan Sequences Through Computerized -- Using Isothermal Titration Calorimetry To Determine Thermodynamic Parameters Of Protein-Glycosaminoglycan Interactions -- Characterizing Protein-Glycosaminoglycan Interactions Using Solution Nmr Spectroscopy.0- Glycosaminoglycan-Protein Interaction Studies Using Fluorescence Spectroscopy -- Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis -- Histochemical Analysis Of Heparan Sulfate 3-O-Sulfotransferase Expression In Mouse Brain -- Keratan Sulfate: Biosynthesis, Structures And Biological Functions -- The Sulfs: Expression, Purification And Substrate Specificity -- The Detection Of Glycosaminoglycans In Pancreatic Islets And Lymphoid Tissues -- Non-Radioactive Glycosyltransferase And Sulfotransferase Assay To Study Glycosaminoglycan Biosynthesis -- Mapping Proteoglycan Functions With Glycosidases -- Cell Substrate Patterning With Glycosaminoglycans To Study Their Biological Roles In The Central -- Analyzing The Role Of Heparan Sulfate Proteoglycans In Axon Guidance In Vivo In Zebrafish -- Murine Models In The Evaluation Of Heparan Sulfate-Based Anticoagulants -- Genetic Approaches In The Study Of Heparan Sulfate Functions In Drosophila -- Measuring Sulfatase Expression And Invasion In Glioblastoma -- Synthesis And Biomedical Applications Of Xylosides -- A Strategic Approach To Identification Of Selective Inhibitors Of Cancer Stem Cells -- Analysis Of The Heavy Chain Modification And Tsg-6 Activity In Pathological Hyaluronan Matrices -- Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration -- Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions -- Role Of Glycosaminoglycans In Infectious Disease -- Isolation And Purification Of Versican And Analysis Of Versican Proteolysis -- Analysis Of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility In Myofibroblast Differentiation.
520 _a Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches.  Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Glycosaminoglycans: Chemistry and Biology serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans.
700 1 _aBalagurunathan, Kuberan
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aNakato, Hiroshi
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDesai, Umesh R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493917150
776 0 8 _iPrinted edition:
_z9781493917136
776 0 8 _iPrinted edition:
_z9781493946969
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1714-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c234366
_d234366