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008			170327s2017 xxu| s |||| 0|eng d
020			_a9781493968077
024	7		_a10.1007/978-1-4939-6807-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Methylation _bMethods and Protocols _cedited by Alexandra Lusser.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 304 páginas) _b62 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1562
505	0		_aLC-MS Analysis of Methylated RNA -- Comparative Analysis of Ribonucleic Acid Digests (CARD) by mass Spectrometry -- Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation -- Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-seq) -- Mapping m6A at Individual-Nucleotide Resolution using Crosslinking and Immunoprecipitation (miCLIP) -- Detection and Quantification of N6-Methyladenosine in Messenger RNA by TLC -- Illustrating the Epitranscriptome at Nucleotide Resolution using Methylation-iCLIP (miCLIP) -- Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing -- Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing -- Analysis of High-Throughput RNA Bisulfite Sequencing Data -- Statistcal Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite -- High-Throughput Mapping of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol) -- RiboMeth-seq: Profiling of 2'-O-Me in RNA -- In Silico Identification of RNA Modifications from High-Throughput Sequencing Data using HAMR -- High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-seq) -- Transcriptome-Wide Mapping of N1-Methyladenosine Methylome -- In Vitro Assays for RNA Methyltransferase Activity -- Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo -- Methylated mRNA Nucleotides as Regulators for Ribosomal Translation -- Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.
520			_aThis volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations. .
700	1		_aLusser, Alexandra _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968053
776	0	8	_iPrinted edition: _z9781493968060
776	0	8	_iPrinted edition: _z9781493983049
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6807-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234367 _d234367