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007			cr nn 008mamaa
008			160503s2016 xxu| s |||| 0|eng d
020			_a9781493936069
024	7		_a10.1007/978-1-4939-3606-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDendritic Cell Protocols _cedited by Elodie Segura, Nobuyuki Onai.
250			_a3rd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 322 páginas) _b68 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1423
505	0		_aReview of Mouse and Human Dendritic Cell Subsets -- In Vitro Generation of Human Xcr1+ Dendritic Cells from Cd34+ Hematopoietic Progenitors -- Derivation and Utilization Of Functional Cd8+ Dendritic Cell Lines -- Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse -- The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and their In Vitro Equivalents -- Dendritic Cell Subsets Purification from Human Tonsils and Lymph Nodes -- Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men -- Isolation of Human Skin Dendritic Cell Subsets -- Isolation of Mouse Dendritic Cell Subsets and Macrophages from Skin -- Isolation of Conventional Dendritic Cells from Mouse Lungs -- Purification of Human Dendritic Cell Subsets from Peripheral Blood -- Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues -- Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay -- Cross-Presentation Assay for Human Dendritic Cells -- Analysis of Intracellular Trafficking of Dendritic Cell Receptors for Antigen Targeting -- Characterization of Dendritic Cell Subsets through Gene Expression Analysis -- In Vivo Ablation of a Dendritic Cell Subset Expressing a Chemokine Receptor, Xcr1 -- In Vivo Analysis Of Intestinal Mononuclear Phagocytes -- In Vivo Imaging Of Cutaneous Dcs In Mice -- Analysis of Dendritic Cell Function by Clec9a-Dtr Transgenic Mice -- Analysis of Dc Functions Using Cd205-Dtr Knock-In Mice -- Generation of Humanized Mice for Analysis of Human Dendritic Cells.
520			_aThe third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
700	1		_aSegura, Elodie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aOnai, Nobuyuki _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936045
776	0	8	_iPrinted edition: _z9781493936052
776	0	8	_iPrinted edition: _z9781493980925
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3606-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234376 _d234376