| 000 | 02643nam a22003495i 4500 | ||
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| 001 | 234380 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020559.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 120327s2012 xxu| s |||| 0|eng d | ||
| 020 | _a9781617797705 | ||
| 024 | 7 |
_a10.1007/978-1-61779-770-5 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aRecombinant Protein Production in Yeast _bMethods and Protocols _cedited by Roslyn M. Bill. |
| 250 | _a1st edition 2012 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c2012 |
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| 300 |
_a1 recurso en línea (XII, 248 páginas) _b47 ilustraciones, 6 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v866 |
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| 520 | _aIn the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host. | ||
| 700 | 1 |
_aBill, Roslyn M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781617797699 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781617797712 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493958719 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-770-5 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2012 | ||
| 999 |
_c234380 _d234380 |
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