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008			141024s2015 xxu| s |||| 0|eng d
020			_a9781493917204
024	7		_a10.1007/978-1-4939-1720-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Pangenomics _bMethods and Protocols _cedited by Alessio Mengoni, Marco Galardini, Marco Fondi.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 298 páginas) _b59 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1231
505	0		_aPulsed Field Gel Electrophoresis and Genome Size Estimates -- Comparative Analyses of Extrachromosomal Bacterial Replicons, Identification of Chromids and Experimental Evaluation of their Indispensability -- Choice of Next Generation Sequencin Pipelines -- The Pyrosequencing Protocol for Bacterial Genomes -- Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing -- The Ilumina-Solexa Sequencing Protocol for Bacterial Genomes -- High-Throughput Phenomics -- Comparative Analysis of Gene Expression: Uncovering Expression Conservation and Divergence between Salmonella enterica serovar Typhimurium strains LT2 and 14028S -- Raw Sequence Data and Quality Control -- Methods for Assembling Reads and Producing Contigs -- Mapping Contigs Using CONTIGuator -- Gene Calling and Bacterial Genome Annotation with BG7 -- Defining Orthologs and Pangenome Size Metrics -- Robust Identification of Orthologues and Paralogues for Microbial Pan-Genomics Using GET_HOMOLOGUES: A Case Study of pIncA/C Plasmids -- Genome-Scale Metabolic Networks Reconstruction -- From Pangenome to Phanphenome and Back -- Genome-Wide Detection of Selection and Other Evolutionary Forces -- The Integrated Microbial Genome Resource of Analysis.
520			_aBacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science.  Thanks to the large diffusion of bacterial genome analysis , Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bioinformatic pipelines for analysis and annotation of sequence data, and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.
700	1		_aMengoni, Alessio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGalardini, Marco _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFondi, Marco _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493917198
776	0	8	_iPrinted edition: _z9781493917211
776	0	8	_iPrinted edition: _z9781493955473
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1720-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234393 _d234393