000			03884nam a22003735i 4500
001			234403
003			ES-VaUE
005			20221220020600.0
007			cr nn 008mamaa
008			100301s2004 xxu| s |||| 0|eng d
020			_a9781592597963
024	7		_a10.1385/1592597963 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aB Cell Protocols _cedited by Hua Gu, Klaus Rajewsky.
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XII, 292 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v271
505	0		_aCharacterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen -- Characterization of B-Cell Maturation in the Peripheral Immune System -- Identification of B-Cell Subsets -- Analysis of B-Cell Life-Span and Homeostasis -- Differentiation of B Lymphocytes From Hematopoietic Stem Cells -- Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System -- Conditional Gene Mutagenesis in B-Lineage Cells -- Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells -- T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences -- of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors -- Immunoglobulin Class Switching -- In Vitro and In Vivo Assays of B-Lymphocyte Migration -- Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo -- B-Cell Signal Transduction -- Isolation of Lipid Rafts From B Lymphocytes -- Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells -- Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice -- Analysis of B-Cell Signaling Using DT40 B-Cell Line -- B-Cell Development and Pre-B-1 Cell Plasticity In Vitro.
520			_aRecent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.
700	1		_aGu, Hua _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRajewsky, Klaus _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375101
776	0	8	_iPrinted edition: _z9781489939609
776	0	8	_iPrinted edition: _z9781588293473
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597963 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2004
999			_c234403 _d234403