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001			234405
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008			151127s2016 xxu| s |||| 0|eng d
020			_a9781493932894
024	7		_a10.1007/978-1-4939-3289-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRecombinant Proteins from Plants _bMethods and Protocols _cedited by Jacqueline MacDonald, Igor Kolotilin, Rima Menassa.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 296 páginas) _b64 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1385
505	0		_aChloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology -- Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors -- Virus-Derived Vectors for the Expression of Multiple Proteins in Plants -- Transient Protein Expression by Agroinfiltration In Lettuce -- Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii -- Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes -- Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation -- Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants -- Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector -- Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots -- Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants -- Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor -- Liquid-Liquid Phase Separation of Oil Bodies From Seeds -- Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract -- Molecular Analyses of Transgenic Plants -- Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response -- Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2 -- A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops -- Generating Transgenic Plants Showing Antibody-Mediated Pathogen Resistance.    .
520			_aThis volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.
700	1		_aMacDonald, Jacqueline _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKolotilin, Igor _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMenassa, Rima _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493932887
776	0	8	_iPrinted edition: _z9781493932900
776	0	8	_iPrinted edition: _z9781493950041
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3289-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234405 _d234405