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001			234407
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008			160908s2016 xxu| s |||| 0|eng d
020			_a9781493964635
024	7		_a10.1007/978-1-4939-6463-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Golgi Complex _bMethods and Protocols _cedited by William J. Brown.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 219 páginas) _b44 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1496
505	0		_a4D Confocal Imaging of Yeast Organelles -- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons -- Imaging Golgi Outposts in Fixed and Living Neurons -- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor -- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells -- Reconstitution of COPI Vesicle and Tubule Formation -- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules -- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures -- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy -- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo -- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures -- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function -- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway -- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle -- The Role of Lysophospholipid Acyltransferases in the Golgi Complex -- Methods to Purify and Assay Secretory Pathway Kinases.
520			_aFocusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques toin vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.iv>.
700	1		_aBrown, William J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964611
776	0	8	_iPrinted edition: _z9781493964628
776	0	8	_iPrinted edition: _z9781493982080
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6463-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234407 _d234407