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001			234412
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007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597454421
024	7		_a10.1007/978-1-59745-442-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicrotubule Protocols _cedited by Jun Zhou.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (X, 305 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v137
505	0		_aMicrotubules -- Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure -- Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts -- Preparation and Characterization of Posttranslationally Modified Tubulins From Artemia franciscana -- Studying the Structure of Microtubules by Electron Microscopy -- Fluorescence Microscopy of Microtubules in Cultured Cells -- Measurements of Stathmin-Tubulin Interaction in Solution -- Analysis of Microtubule Dynamics by Polarized Light -- Visualization of Spindle Behavior Using Confocal Microscopy -- Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila -- Analysis of Tubulin Transport in Nerve Processes -- Analysis of Microtubule-Mediated Intracellular Viral Transport -- Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions -- Screening for Inhibitors of Microtubule-Associated Motor Proteins -- Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy -- Studying Drug-Tubulin Interactions by X-Ray Crystallography -- Characterizing Ligand-Microtubule Binding by Competition Methods -- Methods for Studying Vinca Alkaloid Interactions With Tubulin -- High-Throughput Screening of Microtubule-Interacting Drugs -- Strategies for the Development of Novel Taxol-Like Agents.
520			_aMicrotubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research.
700	1		_aZhou, Jun _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617376917
776	0	8	_iPrinted edition: _z9781588296429
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-442-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c234412 _d234412