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020 _a9781627034814
024 7 _a10.1007/978-1-62703-481-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMouse Models of Innate Immunity
_bMethods and Protocols
_cedited by Irving C. Allen.
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XI, 225 páginas)
_b27 ilustraciones, 20 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1031
505 0 _aConventional Murine Gene Targeting -- Production and Characterization of Humanized Rag2-/-γc-/- Mice -- Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages -- Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages -- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils -- Assessment of Oxidative Metabolism -- Generation and Culture of Mouse Embryonic Fibroblasts -- Primary Ear Fibroblast Derivation from Mice -- Bone Marrow Derived Dendritic Cells -- Measuring T Cell Function in Innate Immune Models -- Bioassay for the Measurement of Type-I Interferon Activity -- Safe and Effective Mouse Foot-Pad Inoculation -- Delayed Type Hypersensitivity Models in Mice -- Mouse Model of Staphylococcus aureus Skin Infection -- Sepsis Induced by Cecal Ligation and Puncture -- Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses -- Mouse Model of Invasive Fungal Infection -- Endotoxin-Induced Uveitis in Rodents -- Bacteria-Mediated Acute Lung Inflammation -- Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome -- Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model -- Bacterial-Mediated Gastrointestinal Inflammation -- Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria -- Characterization of HIV-1 Infection in the Humanized Rag2-/-γc-/- Mouse Model.
520 _aThe innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.
700 1 _aAllen, Irving C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627034821
776 0 8 _iPrinted edition:
_z9781627034807
776 0 8 _iPrinted edition:
_z9781493960361
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-481-4
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2013
999 _c234414
_d234414