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008			160714s2016 xxu| s |||| 0|eng d
020			_a9781493940233
024	7		_a10.1007/978-1-4939-4023-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Wilms' Tumor (WT1) Gene _bMethods and Protocols _cedited by Nicholas Hastie.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 235 páginas) _b42 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1467
505	0		_aWT1 Mutation in Childhood Cancer -- WT1 and Renal Disease -- The Role of WT1 in Embryonic Development and Normal Organ Homeostasis -- Tools and Techniques for WT1-Based Lineage Tracing -- Biological Systems and Methods for Studying WT1 in the Epicardium -- Isolation and Colony Formation of Murine Bone and Bone Marrow Cells -- Isolation and Fluorescent Activated Cell Sorting of Murine WT1-Expressing Adipocyte Precursor Cells -- In Vivo Assays for Assessing the Role of the Wilms' Tumor Suppressor 1 (WT1) in Angiogenesis -- Multiphoton Microscopy for Visualizing Lipids in Tissue -- Function and Regulation of the Wilm's Tumor Suppressor 1 (WT1) Gene in Fish -- Immunofluorescence Staining of WT1 on Sections of Zebrafish Embryos and Larvae -- Fluorescence Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish -- In Vitro Transcription to Study WT1 Function -- Measuring Equilibrium Binding Constants for the WT1-DNA Interaction Using a Filter Binding Assay -- Identifying Direct Downstream Targets: WT1 ChIP-Seq Analysis -- WT1 Associated Protein-Protein Interaction Networks -- Methods to Identify and Validate WT1-RNA Interaction -- Bioinformatic Analysis of Next Generation Sequencing Data to Identify WT1 Associated Differential Gene and Isoform Expression -- Immunotherapy Targeting WT1: Designing a Protocol for WT1 Peptide-Based Cancer Vaccine.
520			_aThis volume provides a thorough overview of the Wilms' Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools. The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, The Wilms' Tumour (WT1) Gene: Methods and Protocols is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research. .
700	1		_aHastie, Nicholas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493940219
776	0	8	_iPrinted edition: _z9781493940226
776	0	8	_iPrinted edition: _z9781493981564
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-4023-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234415 _d234415