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008			101116s2011 xxu| s |||| 0|eng d
020			_a9781607619741
024	7		_a10.1007/978-1-60761-974-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTransgenic Mouse Methods and Protocols _cedited by Marten H. Hofker, Jan van Deursen.
250			_a2nd edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 352 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v693
505	0		_aIntroduction: Strategies for developing genetically modified mice -- Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection -- Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos -- Cryopreservation of mouse spermatozoa and In vitro fertilization -- Autopsy and histological analysis of the transgenic mouse -- Transgene design -- Inducible Transgenic Mouse Models -- Lentiviral transgenesis -- Transgenesis in Mouse Embryonic Stem Cells -- Engineering BAC Reporter Gene Constructs for Mouse Transgenesis -- Targeting Vector Construction Through Recombineering -- Generating Conditional Knockout Mice -- Hypomorphic mice -- MICER Targeting Vectors for Manipulating the Mouse Genome -- Knockin approaches -- Generation of a series of knockin alleles using RMCE in ES cells -- Selection of targeted mutants from a library of randomly mutagenized ES cells -- Generation of genetically modified rodents using random ENU mutagenesis -- Bone marrow transplantations to study gene function in hematopoietic cells -- Adenovirus Mediated Gene Transfer.
520			_aThe generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.
700	1		_aHofker, Marten H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_avan Deursen, Jan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607619734
776	0	8	_iPrinted edition: _z9781607619758
776	0	8	_iPrinted edition: _z9781493956760
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-974-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234419 _d234419