000 02547nam a22003495i 4500
001 234421
003 ES-VaUE
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008 120329s2012 ja | s |||| 0|eng d
020 _a9784431539933
024 7 _a10.1007/978-4-431-53993-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPatch Clamp Techniques
_bFrom Beginning to Advanced Protocols
_cedited by Yasunobu Okada.
250 _a1st edition 2012
264 1 _aTokyo :
_bSpringer Japan :
_bImprint: Springer,
_c2012
300 _a1 recurso en línea (XI, 439 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aSpringer Protocols Handbooks
_x1949-2456
520 _aChannels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
700 1 _aOkada, Yasunobu
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9784431539926
776 0 8 _iPrinted edition:
_z9784431539940
776 0 8 _iPrinted edition:
_z9784431561057
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-4-431-53993-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2012
999 _c234421
_d234421