000			04053nam a22003615i 4500
001			234423
003			ES-VaUE
005			20221220020601.0
007			cr nn 008mamaa
008			170424s2017 xxu| s |||| 0|eng d
020			_a9781493968664
024	7		_a10.1007/978-1-4939-6866-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicroRNA Detection and Target Identification _bMethods and Protocols _cedited by Tamas Dalmay.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 304 páginas) _b84 ilustraciones, 75 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1580
505	0		_aImproved denaturation of Small RNA Duplexes and its Application for Northern-blotting -- High throughput RT-qPCR for the Analysis of Circulating microRNAs -- Genome-wide Comparison of Next Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum -- Small RNA Profiling by Next Generation Sequencing using High Definition Adapters -- Surface Acoustic Wave Lysis and Lon-exchange Membrane Quantification of Exosomal microRNA -- Droplet Microfluidic Device Fabrication and use for Isothermal Amplification and Detection of microRNA -- Interrogation of Functional miRNA-target Interactions by CRISPR/Cas9 Genome Engineering -- Cell-free Urinary microRNAs Expression in small-scale Experiments -- Peptide-based Isolation of Argonaute Protein Complexes using Ago-APP -- Predicting functional microRNA-mRNA interactions.-Computational and Experimental Identification of Tissue-specific microRNA Targets -- sRNAtoolboxVM: Small RNA Analysis in a Virtual Machine -- An Assessment of the Next Generation of Animal miRNA Target Prediction Algorithms -- The UEA Small RNA Workbench: A Suite of Computational Tools for Small RNA Analysis -- Prediction of miRNA-mRNA Interactions using miRGate -- Detection of microRNAs using Chip-based QuantStudio 3D Digital PCR -- MiRNA Quantitation with Microelectrode Sensors Enabled by Enzymeless Electrochemical Signal Amplification -- A Robust Protocol to Quantify Circulating Cancer Biomarker microRNAs -- MicroRNAs, Regulatory Networks and Comorbidities: Decoding Complex Systems -- Label-free Direct Detection of MiRNAs with Poly-silicon Nanowire Biosensors. .
520			_aThis volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aDalmay, Tamas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968640
776	0	8	_iPrinted edition: _z9781493968657
776	0	8	_iPrinted edition: _z9781493983223
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6866-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234423 _d234423