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008			150512s2015 xxu| s |||| 0|eng d
020			_a9781493927302
024	7		_a10.1007/978-1-4939-2730-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Scaffolds _bMethods and Protocols _cedited by Luc Ponchon.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIII, 231 páginas) _b61 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1316
505	0		_aA Method to Predict the 3D Structure of an RNA Scaffold -- Post-crystallization Improvement of RNA Crystal Diffraction Quality -- Expression and Purification of RNA-protein Complexes in Escherichia coli -- Production of Homogeneous Recombinant RNA using a tRNA Scaffold and Hammerhead Ribozymes -- In vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold -- Detection of RNA-Protein Interactions using Tethered RNA Affinity Capture -- A Universal Method for Labeling Native RNA in Live Bacterial Cells -- Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors -- RNA Scaffold: Designed To Co-Localize Enzymes -- Artificial Ligase Ribozymes Isolated by a "Design And Selection" Strategy -- Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach -- Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells -- Design and Characterisation of Topological Small RNAs -- Folding RNA-Protein Complex into Designed Nanostructures -- Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60˚ to 90˚ or 108˚ -- RNA-Mediated CdS Based Nanostructures -- An Effective Method for Specific Gene Silencing in Escherichia coli using Artificial Small RNA.
520			_aThis volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.
700	1		_aPonchon, Luc _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493927296
776	0	8	_iPrinted edition: _z9781493927319
776	0	8	_iPrinted edition: _z9781493955893
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2730-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234426 _d234426