000			04017nam a22003735i 4500
001			234438
003			ES-VaUE
005			20221220020602.0
007			cr nn 008mamaa
008			120621s2012 xxu| s |||| 0|eng d
020			_a9781617799310
024	7		_a10.1007/978-1-61779-931-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAntibody Methods and Protocols _cedited by Gabriele Proetzel, Hilmar Ebersbach.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (X, 325 páginas) _b55 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v901
505	0		_aAntigen Presentation for the Generation of Binding Molecules -- Recombinant Antibodies and In Vitro Selection Technologies -- Phage Display -- Generation of Human Fab Libraries for Phage Display -- Selection of Human Fab Libraries by Phage Display -- Ribosome Display -- Hybridoma Technology for the Generation of Monoclonal Antibodies -- The Application of Transgenic Mice for Therapeutic Antibody Discovery -- Production of Human or Humanized Antibodies in Mice -- Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies -- Kinetic Screening in the Antibody Development Process -- Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection -- Determination of Antibody Glycosylation by Mass Spectrometry -- Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format -- PEGylation of Antibody Fragments for Half-Life Extension -- Bispecific Antibody Derivatives Based on Full-Length IgG Formats -- Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells -- Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function -- Class-Specific Effector Functions of Therapeutic Antibodies.
520			_aThe rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.
700	1		_aProetzel, Gabriele _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aEbersbach, Hilmar _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617799327
776	0	8	_iPrinted edition: _z9781617799303
776	0	8	_iPrinted edition: _z9781493959303
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-931-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c234438 _d234438