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020 _a9781603272483
024 7 _a10.1007/978-1-60327-248-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGene Therapy Protocols
_bVolume 2: Design and Characterization of Gene Transfer Vectors
_cedited by Joseph LeDoux.
250 _a3rd edition 2008
264 1 _aTotowa, NJ
_bHumana Press
_c2008
300 _a1 recurso en línea (XII, 313 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v434
505 0 _aPurification of Retrovirus Particles Using Heparin Affinity Chromatography -- Scaleable Purification of Adenovirus Vectors -- Quantifying the Titer and Quality of Adenovirus Stocks -- Chromatography-Based Purification of Adeno-Associated Virus -- Spectroscopic Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery Systems -- Real-Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments -- Production of Lentiviruses Displaying "Early-Acting" Cytokines for Selective Gene Transfer into Hematopoietic Stem Cells -- Fiber-modified Adenoviruses for Targeted Gene Therapy -- PEGylated Adenovirus for Targeted Gene Therapy -- Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties -- Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes -- Reducing the Genotoxic Potential of Retroviral Vectors -- Evaluation of Promoters for Use in Tissue-Specific Gene Delivery -- Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cells -- Regulated Expression of Adenoviral Vectors-Based Gene Therapies -- Liver-Directed Gene Therapy Using the Sleeping Beauty Transposon System -- Generation and Functional Analysis of Zinc Finger Nucleases -- Conditional Gene Expression and Knockdown Using Lentivirus Vectors Encoding shRNA -- Nanoparticle-Mediated Gene Delivery to the Lung -- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype -- In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription -- Efficient Retroviral Gene Transfer to Epidermal Stem Cells.
520 _aIn Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today's technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.
700 1 _aLeDoux, Joseph
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781603272476
776 0 8 _iPrinted edition:
_z9781627039642
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-248-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2008
999 _c234440
_d234440