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020 _a9781592595426
024 7 _a10.1385/0896033287
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Cell Electroporation And Electrofusion Protocols
_cedited by Jac A. Nickoloff.
250 _a1st edition 1995
264 1 _aTotowa, NJ
_bHumana Press
_c1995
300 _a1 recurso en línea (XIV, 206 páginas)
_b15 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v55
505 0 _aTheory and Instrumentation -- Electroporation Theory -- Effects of Pulse Length and Strength on Electroporation Efficiency -- Instrumentation -- Electroporation Protocols -- Electroporation of Agrobacterium tumefaciens -- Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii -- Pollen Electrotransformation in Tobacco -- Electroporation of Tobacco Leaf Protoplasts Using Plasmid DNA or Total Genomic DNA -- Electroporation of Brassica -- Transformation of Maize by Electroporation of Embryos -- Transient Gene Expression Analysis in Electroporated Maize Protoplasts -- Reporter Genes and Transient Assays for Plants -- Electrofusion Protocols -- Electrofusion of Plant Protoplasts -- Protoplast Electrofusion and Regeneration in Potato -- Polymer-Supported Electrofusion of Protoplasts.
520 _aGene transfer is an essential technology for improving our under­ standing of gene structure and function. Although there are many meth­ ods by which DNA may be introduced into cells-including heat and chemical treatments, and microinjection-electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi­ ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in­ cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna­ tives to electric field-based methods, citing the advantages and limita­ tions of the various methods for achieving specific goals.
700 1 _aNickoloff, Jac A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489940803
776 0 8 _iPrinted edition:
_z9781489940797
776 0 8 _iPrinted edition:
_z9780896033283
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033287
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1995
999 _c234445
_d234445