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020			_a9781592590520
024	7		_a10.1385/1592590527 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Toxins _bMethods and Protocols _cedited by Otto Holst.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XI, 379 páginas) _b27 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v145
505	0		_aBacterial Protein Toxins -- Purification of Clostridium botulinum Type A Neurotoxin -- Shiga Toxins -- Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins -- Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins -- Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins -- Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins -- The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins -- Site-Directed Spin Labeling of Proteins -- Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers -- Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance -- ADP-Ribosylation of ?-Gi Proteins by Pertussis Toxin -- Phage Libraries for Generation of Anti-Botulinum scFv Antibodies -- T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors -- In Vitro Physiological Studies on Clostridial Neurotoxins -- The Biology of Endotoxin -- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides -- Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides -- Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates -- Electrophysiological Measurements on Reconstituted Outer Membranes.
520			_aThe interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial toxins need to be acquainted with all these disciplines in order to work effectively in the field. To date, there has been no published collection offering detailed descr- tions of the techniques and methods needed by researchers operating across the field'sdiverse areas. The present volume Bacterial Toxins: Methods and Pro- cols, is intended to fill this gap. Bacterial Toxins: Methods and Protocols consists of two sections: one on protein toxins (15 chapters) and one on endotoxins (5 chapters). Each s- tion is introduced by an overview article (Chapters 1 and 16). The protocols collected represent state-of-the-art techniques that each have high impact on future bacterial toxin research. All methods are described by authors who have regularly been using the protocol in their own laboratories. Included in each chapter is a brief introduction to the method being described.
700	1		_aHolst, Otto _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489941381
776	0	8	_iPrinted edition: _z9780896036048
776	0	8	_iPrinted edition: _z9781489941374
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590527 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c234446 _d234446