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008			101130s2011 xxu| s |||| 0|eng d
020			_a9781603271981
024	7		_a10.1007/978-1-60327-198-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCharacterization of Nanoparticles Intended for Drug Delivery _cedited by Scott E. McNeil.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 269 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v697
505	0		_aUnique Benefits of Nanotechnology to Drug Delivery and Diagnostics -- Challenges for Nanoparticle Characterization -- Considerations when submitting nano-therapeutics to FDA/CDER for regulatory review -- Measuring the Hydrodynamic Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering -- Characterization of Nanoparticles by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry -- Zeta Potential Measurement -- Size Measurement of Nanoparticles using Atomic Force Microscopy -- Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization.- SEM X-ray Microanalysis of Nanoparticles Present in Tissue or Cultured Cell Thin Sections -- Detecting and Measuring Free Gadolinium in Nanoparticles for MRI Imaging -- Lipid Component Quantitation by Thin Layer Chromatography -- Detection and Quantitative Evaluation of Endotoxin Contamination in Nanoparticle Formulations by LAL-based Assays -- Analysis of microbial contamination in nanoparticle formulations -- Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture -- Quantitation of Nanoparticles in Serum Matrix by Capillary Electrophoresis -- Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells -- Monitoring Nanoparticle-Treated Hepatacarcinoma Cells for Apoptosis - Detecting Reactive Oxygen Species in Primary Hepatocytes Treated with Nanoparticles -- Assay to Detect Lipid Peroxidation Upon Exposure to Nanoparticles -- Monitoring Glutathione Homeostasis in Nanoparticle-Treated Hepatocytes -- Autophagy Monitoring Assay: Qualitative Analysis of MAP LC3-I to II Conversion by Immunoblot -- Monitoring Lysosomal Activity in Nanoparticle-Treated Cells -- Method for Analysis of Nanoparticle Hemolytic Properties in Vitro -- Method for In Vitro Analysis of Nanoparticle Thrombogenic Properties -- Qualitative Analysis of Total Complement Activation by Nanoparticles -- Method for Analysis of Nanoparticle Effects on Cellular Chemotaxis -- In Vitro Analysis of Nanoparticle Uptake by Macrophages Using Chemiliuminescence.
520			_aIn recent years, there have been many exciting breakthroughs in the application of nanotechnology to medicine. In Characterization of Nanoparticles Intended for Drug Delivery, expert researchers explore the latest advances in the field, providing a set of basic methods for the characterization of nanomaterials for medical use. Chapters provide methods to characterize the physiochemical properties (size, aggregation, and surface chemistry) and in vitro immunological and biological characteristics of nanomaterials. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and informative, Characterization of Nanoparticles Intended for Drug Delivery is an essential survey of methods that are crucial to the preclinical characterization of nanomedicines.
700	1		_aMcNeil, Scott E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603271974
776	0	8	_iPrinted edition: _z9781607619086
776	0	8	_iPrinted edition: _z9781493961139
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-198-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234449 _d234449