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008			100301s1997 xxu| s |||| 0|eng d
020			_a9781592595679
024	7		_a10.1385/089603495X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aReceptor Signal Transduction Protocols _cedited by R. A. J. Challiss.
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XIII, 275 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v83
505	0		_aMethods for Characterizing Receptors -- Radioligand-Binding Methods for Membrane Preparations and Intact Cells -- Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding -- Approaches to the Stable Transfection of G Protein-Coupled Receptors -- Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels -- The Generation of Receptor-Selective Antibodies -- In Situ Hybridization -- lmmunocytochemical Methods for Investigating Receptor Localization -- Methods for Studying Receptor Coupling Mechanisms -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes -- Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTP?S Binding -- Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes -- Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling -- Identification and Quantitation of G Protein ?-Subunits -- Covalent Modification of G Proteins by Affinity Labeling -- Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection -- Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C -- Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons -- Methods for Investigating the Regulation of Receptor Function -- Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation -- Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation -- Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells -- Regulation of Receptor Expression.
520			_aR. A. J. Challiss assembles in Receptor Signal Transduction Protocols an outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. These powerful methods emphasize the regulation of G-protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G-proteins, and approaches to studying how receptor expression and function is regulated. Designed to be easily reproducible, the protocols offered include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G-protein function, antisense methods for defining receptor-effector coupling pathways, and many more. Receptor Signal Transduction Protocols blends authoritative descriptions and applications of well-established methodologies with many novel techniques at the cutting edge of signal transduction research. The coverage cuts across disciplines and allows those already well-versed in one area to expand their repertoire of potent methods and broaden their research horizons. Every researcher interested in signal transduction today will find this richly practical guide indispensable.
700	1		_aChalliss, R. A. J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942838
776	0	8	_iPrinted edition: _z9780896034952
776	0	8	_iPrinted edition: _z9781489942821
776	0	8	_iPrinted edition: _z9780896034181
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/089603495X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1997
999			_c234455 _d234455