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008			141020s2015 xxu| s |||| 0|eng d
020			_a9781493917525
024	7		_a10.1007/978-1-4939-1752-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMethods in Membrane Lipids _cedited by Dylan M. Owen.
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 327 páginas) _b43 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1232
505	0		_aIntroduction to Membrane Lipids -- Introduction: Membrane properties (Good) for Life -- Introduction To Fluorescence Probing Of Biological Membranes -- The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs) -- Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells -- Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning -- Asymmetric Giant Lipid Vesicle Fabrication -- Cholesterol Depletion using Methyl-b-cyclodextrin -- A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems -- Imaging Membrane Order using Environmentally Sensitive Fluorophores -- 3D Super-resolution Imaging by Localisation Microscopy -- Electron Microscopy Methods for Studying Plasma Membranes -- Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy -- Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order -- Scanning Fluorescence Correlation Spectroscopy on Biomembranes -- X-ray Diffraction of Lipid Model Membranes -- Solid State NMR of Lipid Model Membranes -- Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications -- The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells -- Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations -- Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers.                                                                                                                                                                        .
520			_aThis volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico.  Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative  and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.
700	1		_aOwen, Dylan M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493917532
776	0	8	_iPrinted edition: _z9781493917518
776	0	8	_iPrinted edition: _z9781493953998
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1752-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234456 _d234456