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008			170722s2017 xxu| s |||| 0|eng d
020			_a9781493971428
024	7		_a10.1007/978-1-4939-7142-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAcute Myeloid Leukemia _bMethods and Protocols _cedited by Paolo Fortina, Eric Londin, Jason Y. Park, Larry J. Kricka.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 278 páginas) _b46 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1633
505	0		_aMolecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia -- Cytochemical Staining -- Immunohistochemistry in Acute Myeloid Leukemia -- Immunophenotyping by Multiparameter Flow Cytometry -- Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells -- Design and Application of Multiplex-PCR-Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies -- AMLProfiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia -- Microsphere-Based Assessment of DNA Methylation for AML Prognosis -- Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS -- Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts -- Targeted Next-Generation Sequencing of Acute Leukemia -- Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples -- A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and its Mutation -- Measurement of Oncometabolites D-2-Hydroxyglutaric Acid and L-2-Hydroxyglutaric Acid -- Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukaemia -- Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer -- Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia.
520			_aThis volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.
700	1		_aFortina, Paolo _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLondin, Eric _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPark, Jason Y _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKricka, Larry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971404
776	0	8	_iPrinted edition: _z9781493971411
776	0	8	_iPrinted edition: _z9781493983964
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7142-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234460 _d234460