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020 _a9781592599899
024 7 _a10.1385/1592599893
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlacenta and Trophoblast
_bMethods and Protocols, Volume II
_cedited by Michael J. Soares, Joan S. Hunt.
250 _a1st edition 2006
264 1 _aTotowa, NJ
_bHumana Press
_c2006
300 _a1 recurso en línea (XXI, 452 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v122
505 0 _aPlacenta and Trophoblast: Methods and Protocols: Overview II -- Analysis of Trophoblast Invasion -- In Vivo Analysis of Trophoblast Cell Invasion in the Human -- In Vitro Analysis of Trophoblast Invasion -- An In Vitro Model of Trophoblast Invasion of Spiral Arteries -- Analysis of Uteroplacental Immune Cells and Their Functions -- In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells -- Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface -- Methods for Isolation of Cells from the Human Fetal-Maternal Interface -- In Vitro Models for Studying Human Uterine and Placental Macrophages -- Macrophage-Trophoblast Interactions -- Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon -- Analysis of the Soluble Isoforms of HLA-G -- Analysis of Placental Function -- In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport -- In Vitro Models for Studying Trophoblast Transcellular Transport -- In Vitro Methods for Studying Human Placental Amino Acid Transport -- In Vitro Methods for Studying Human Placental Amino Acid Transport -- Methods for Investigating Placental Fatty Acid Transport -- Heterologous Expression Systems for Studying Placental Transporters -- Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures -- Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells -- Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands -- Bacterial Expression of Prolactin Family Proteins -- Analysis of Placental Regulation of Hematopoiesis -- Methods for Studying Interferon Tau Stimulated Genes -- ANALYSIS OF PLACENTA ADAPTATION TO DISEASE -- Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia -- In Vivo Rat Model of Preeclampsia -- A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice -- Working with Oxygen and Oxidative Stress In Vitro -- Hypobaric Hypoxia as a Tool to Study Pregnancy-Dependent Responses at the Maternal-Fetal Interface -- Infection With Listeria monocytogenes as a Probe for Placental Immunological Function.
520 _aPlacental research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 2 provides readily reproducible protocols for studying trophoblast invasion, maternal immune cell responses to pregnancy, and the hormone-producing capabilities of the placenta. There are also methods to investigate the barrier function of the placenta, its role in regulating the transport of nutrients and wastes, and the adaptations of trophoblast cells and the placenta to disease. Highlights include techniques to analyze trophoblast cell interactions with the uterine vasculature and immune cells, and to the endocrine functions of the placenta. A companion first volume concentrates on methods for investigate embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.
700 1 _aSoares, Michael J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHunt, Joan S
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781588296085
776 0 8 _iPrinted edition:
_z9781627038638
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599893
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2006
999 _c234461
_d234461