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020 _a9781493925193
024 7 _a10.1007/978-1-4939-2519-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMammary Stem Cells
_bMethods and Protocols
_cedited by Maria del Mar Vivanco.
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (X, 275 páginas)
_b58 ilustraciones, 45 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1293
505 0 _aBreast Cancer Stem Cells: Current Advances and Clinical Implications -- A Protocol for Studying Embryonic Mammary Progenitor Cells During Mouse Mammary Primordial Development in Explant Culture -- FACS Sorting Mammary Stem Cells -- Side Population -- Single Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing -- Shotgun Proteomics on Tissue Specimens Extracted with Acid Guanidinium-Thiocyanate-Phenol-Chloroform -- Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments -- Lentiviral Transduction of Mammary Epithelial Cells -- The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads -- Humanization of the Mouse Mammary Gland -- Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles -- Modeling the Breast Cancer Bone Metastatic Niche in Complex Three Dimensional Co-Cultures -- Mammary Cancer Stem Cells Re-Initiation Assessment at the Metastatic Niche: The Lung and Bone -- Nanomechanical Characterization of Living Mammary Tissues by Atomic Force Microscopy -- Mathematical Modeling as a Tool to Understand Cell Self-Renewal and Differentiation -- Mammary Stem Cells: A Clinician´s View.
520 _aThe identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.
700 1 _aVivanco, Maria del Mar
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493925186
776 0 8 _iPrinted edition:
_z9781493925209
776 0 8 _iPrinted edition:
_z9781493948499
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2519-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c234467
_d234467