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001			234470
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007			cr nn 008mamaa
008			160531s2016 xxu| s |||| 0|eng d
020			_a9781493935390
024	7		_a10.1007/978-1-4939-3539-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEndocannabinoid Signaling _bMethods and Protocols _cedited by Mauro Maccarrone.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 285 páginas) _b75 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1412
505	0		_aNeed for Methods to Investigate Endocannabinoid Signaling -- Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues -- Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS -- Analysis of Omega-3 Fatty Acid Derived N-acylethanolamines in Biological Matrices -- Assay of CB1 Receptor Binding -- The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells -- Assay of TRPV1 Receptor Signaling -- A Functional Assay for GPR55: Envision Protocols -- The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells -- Assay of GTPγS Binding in Autoradiography -- Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors -- Assay of NAT Activity -- Assay of NAPE-PLD Activity -- Assay of FAAH Activity -- Assay of NAAA Activity -- Assay of DAGLα/β Activity -- Assay of Monoacylglycerol Lipase Activity -- A Sensitive and Versatile Fluorescent Activity Assay for ABHD6 -- A Sensitive and Versatile Fluorescent Activity Assay for ABHD12 -- Assay of Endocannabinoid Uptake -- Assay of Endocannabinoid Oxidation by Cyclooxygenase-2 -- Oxygenation of Anandamide by Lipoxygenases -- Assay of Endocannabinoid Oxidation by Cytochrome P450 -- Assessing Gene Expression of the Endocannabinoid System -- Western Blotting of the Endocannabinoid System -- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells -- Measuring ECS Interaction with Biomembranes -- Visualization of Endocannabinoids in the Cell.
520			_aThis volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest towards the manifold activities of eCBs, this book discusses the chemical, biochemical, and molecular biological assays, and activity of distinct elements of the ECS. These include membrane, nuclear receptors, biosynthetic and hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocols is a valuable resource and will help chemists, drug designers, biochemists, molecular biologists, cell biologists, pharmacologists, and (electro) physiologists navigate the mare magnum of endocannabinoid research.
700	1		_aMaccarrone, Mauro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493935376
776	0	8	_iPrinted edition: _z9781493935383
776	0	8	_iPrinted edition: _z9781493980710
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3539-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234470 _d234470