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020 _a9781603272797
024 7 _a10.1007/978-1-60327-279-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aAntibiotic Resistance Protocols
_bSecond Edition
_cedited by Stephen H. Gillespie, Timothy D. McHugh.
250 _a2nd edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (XII, 236 páginas)
_b37 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v642
505 0 _aEpidemiology and Population Genetics -- The RM Test for Determining Methicillin-Resistant Staphylococcus aureus Lineages -- Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx -- Genomics and Gene Expression -- Methods to Determine Antibiotic Resistance Gene Silencing -- Studying Gene Induction of Glycopeptide Resistance Using Gene Swapping -- Real-Time PCR Methods to Study Expression of Genes Related to Hypermutability -- Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial Compounds -- Fitness Mutation and Physiology -- Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity -- Measurements of Heme Levels and Respiration Rate in Salmonella enterica Serovar Typhimurium -- Methods to Determine Fitness in Bacteria -- Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosis -- Detection of Resistance -- Identification of Efflux-Mediated Multi-drug Resistance in Bacterial Clinical Isolates by Two Simple Methods -- Evaluation of Efflux Activity of Bacteria by a Semi-automated Fluorometric System -- Measuring the Activity of Active Efflux in Gram-Negative Bacteria -- Rapid Characterization of ?-Lactamases by Multiplex PCR -- Rapid Methods for Testing Inhibitors of Mycobacterial Growth -- Use of Biofilm Model Systems to Study Antimicrobial Susceptibility -- Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing®.
520 _aSince the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.
700 1 _aGillespie, Stephen H
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMcHugh, Timothy D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607617181
776 0 8 _iPrinted edition:
_z9781603272780
776 0 8 _iPrinted edition:
_z9781493960729
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-279-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2010
999 _c234477
_d234477