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001			234479
003			ES-VaUE
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007			cr nn 008mamaa
008			140218s2014 xxu| s |||| 0|eng d
020			_a9781627037556
024	7		_a10.1007/978-1-62703-755-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aArtificial Riboswitches _bMethods and Protocols _cedited by Atsushi Ogawa.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (X, 289 páginas) _b75 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1111
505	0		_aComputational Design of RNA Libraries for in vitro Selection of Aptamers -- In vitro Selection of RNA Aptamers for a Small-Molecule Dye -- Development of Photoswitchable RNA Aptamer-Ligand Complexes -- Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag -- Artificial Riboswitch Selection: A FACS-Based Approach -- FRET-Based Optical Assay for Selection of Artificial Riboswitches -- Portable Two-Way Riboswitches: Design and Engineering -- Generation of Orthogonally Selective Bacterial Riboswitches By Targeted Mutagenesis and in vivo Screening -- Dual Genetic Selection of Synthetic Riboswitches in Escherichia coli -- Nucleotide Kinase-Based Selection System for Genetic Switches -- Measuring Riboswitch Activity in vitro and in Artificial Cells with Purified Transcription-Translation Machinery -- Rational Design of Artificial ON-Riboswitches -- Engineering Protein-Responsive mRNA Switch in Mammalian Cells -- Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators -- In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP -- Dual-Selection for Evolution of in vivo Functional Aptazymes as Riboswitch Parts -- In vivo Screening for Aptazyme-Based Bacterial Riboswitches -- Engineered Riboswitch as Gene-Regulatory Platform for Reducing Antibiotic Resistance -- Construction of Ligand-Responsive microRNAs that Operate Through Inhibition of Drosha Processing -- A Three-Dimensional Design Strategy for a Protein-Responsive shRNA Switch.
520			_aArtificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.
700	1		_aOgawa, Atsushi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627037563
776	0	8	_iPrinted edition: _z9781627037549
776	0	8	_iPrinted edition: _z9781493963300
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-755-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234479 _d234479