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020 _a9781493912803
024 7 _a10.1007/978-1-4939-1280-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aQuantum Dots: Applications in Biology
_cedited by Adriana Fontes, Beate Saegesser Santos.
250 _a2nd edition 2014
264 1 _aNew York, NY
_bSpringer International Publishing
_c2014
300 _a1 recurso en línea (XI, 258 páginas)
_b76 ilustraciones, 65 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1199
505 0 _aQuantum Dots as Biophotonics Tools -- Photoligation Combined with Zwitterion-Modified Lipoic Acid Ligands Provides Compact and Biocompatible Quantum Dots -- Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence -- Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum Dots -- Quantum Dots-Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging -- Measuring the Hydrodynamic Radius of Quantum Dots by Fluorescence Correlation Spectroscopy -- Quantum Dots Fluorescence Quantum Yield Measured by Thermal Lens Spectroscopy -- Semiquantitative Fluorescence Method for Bioconjugation Analysis -- Assembly, Characterization and Delivery of Quantum Dot Labeled Biotinylated Lipid Particles -- Oriented Conjugation of Single-Domain Antibodies and Quantum Dots -- Functional Integration of Quantum Dot Labeled Mesenchymal Stem Cells in a Cardiac Microenvironment -- In Vitro Approaches to Assessing the Toxicity of Quantum Dots -- Basics for the Preparation of Quantum Dots and their Interactions with Living Cells -- In Vivo Approaches to Assessing the Toxicity of Quantum Dots -- Quantum Dots for Imaging Neural Cells In Vitro and In VivoA Short-Term Inhalation Study Protocol - Designed for Testing of Toxicity and Fate of Nanomaterials -- Quantitative Measurement of Proteolytic Rates with Quantum Dot-Peptide Substrate Conjugates and Förster Resonance Energy Transfer -- Solid-Phase Supports for the in situ Assembly of Quantum Dot-FRET Hybridization Assays in Microfluidics.
520 _aIn Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots(QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs´ preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals, and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research.
700 1 _aFontes, Adriana
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSantos, Beate Saegesser
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493912810
776 0 8 _iPrinted edition:
_z9781493912797
776 0 8 _iPrinted edition:
_z9781493954155
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1280-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2014
999 _c234484
_d234484