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001			234485
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008			131016s2014 xxu| s |||| 0|eng d
020			_a9781627036436
024	7		_a10.1007/978-1-62703-643-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Cell Morphogenesis _bMethods and Protocols _cedited by Viktor Žárský, Fatima Cvrčková.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 285 páginas) _b43 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1080
505	0		_aEssential Methods of Plant Sample Preparation for Light Microscopy -- Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy -- Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues -- Automated Microscopy in Forward Genetic Screening of Arabidopsis -- Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections -- Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells -- Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM -- Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces -- Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope -- Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX -- Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy -- High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy -- Reconstructing Plant Cells in 3D by Serial Section Electron Tomography -- Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy -- Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections -- Live Cell Imaging of Arabidopsis Root Hairs -- Morphological Analysis of Cell Growth Mutants in Physcomitrella -- Plant Cell Lines in Cell Morphogenesis Research -- Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes -- Lab-on-a-Chip for Studying Growing Pollen Tubes -- Laser Microdissection of Plant Cells -- Optical Trapping in Plant Cells -- Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery.
520			_a Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on  classical light microscopy  and  histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized  electron microscopy  techniques, and methods  used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell  morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by  expert researchers in the field and  include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
700	1		_aŽárský, Viktor _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aCvrčková, Fatima _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627036443
776	0	8	_iPrinted edition: _z9781627036429
776	0	8	_iPrinted edition: _z9781493960323
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-643-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234485 _d234485